Identification of Nosema ceranae in the Valparaíso District, Chile

  1. J Bravo
  2. V Carbonell
  3. JT Valdebenito
  4. C Figueroa
  5. CE Valdovinos
  6. R Martín-Hernández
  7. M Higes
  8. C Delporte
Revista:
Archivos de Medicina Veterinaria

ISSN: 0301-732X,  0717-6201

Año de publicación: 2014

Volumen: 46

Número: 3

Páginas: 487-491

Tipo: Artículo

DOI: 10.4067/S0301-732X2014000300021 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Archivos de Medicina Veterinaria

Objetivos de desarrollo sostenible

Resumen

This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees were macerated and analysed by phase contrast microscopy at 40x. Then, DNA was extracted and the gene that encodes for 16SrRNA of Nosema apis and N. ceranae was amplified by multiplex PCR. 100% of the samples were infected by N. ceranae, while N. apis was not detected. During the period of study 2,915 colonies died in the Valparaíso Region which is 47.3% of the total hives of the Region. The loss of beehives during this period in the V Region of Chile could be related to either the lack of training in management and health monitoring of the colonies or the presence of N. ceranae.

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